Język metadanych
Construction of plasmid bicistronic vectors and their application for in vitro transfection
Inny tytuł:Construction of plasmid bicistronic vectors and their application for in vitro transfection
Twórca:Kucharzewska, Paulina ; Zagórska, Anna ; Leja, Justyna ; Jaźwa, Agnieszka ; Gozdecka, Małgorzata ; Józkowicz, Alicja ; Dulak, Józef
Wydawca:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Data wydania/powstania: Temat i słowa kluczowe: Abstrakt:Internal ribosome entry site (IRES) sequences, which stand for the basic element of cap 5’-independent translation, are currently widely used to coexpress heterologous genes from one plasmid. In this study construction of four bicistronic plasmids containing IRES and application of these vectors for transfection of in vitro cultured cells were described. The obtained data show that constructed bicistronic plasmids are very efficient in vitro in terms of simultaneous expression of fibroblast growth factor-4 (FGF-4) and vascular endothelial growth factor (VEGF) or one of these factors and green fluorescent protein (GFP) from one plasmid. Interestingly, expression of two genes, although simultaneous, is not equal. It has turned out that IRES-dependent mRNA translation is less efficient than cap 5’-dependent translation of the first gene, which should be taken into account during construction of bicistronic plasmids.
Czasopismo/Seria/cykl:Biotechnologia, vol.78, 3 (2007)-.
Tom: Zeszyt: Strona pocz.: Strona końc.: Typ zasobu: Szczegółowy typ zasobu: Format: Identyfikator zasobu: Źródło:Library of Institute of Bioorganic Chemistry PAS
Język: Język streszczenia: Zakres czasowy: Prawa:Creative Commons Attribution BY-SA 4.0 license
Zasady wykorzystania: Digitalizacja:Institute of Bioorganic Chemistry of the Polish Academy of Science
Lokalizacja oryginału:Institute of Bioorganic Chemistry of the Polish Academy of Science
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