Title:

Przeszukiwanie bazy danych EST w celu zidentyfikowania pełnej sekwencji cDNA genu kodującego akwaporynę Pharbitis nil Choisy (PnPIPl)

Subtitle:

Screening of the EST database for identification of the complete cDNA sequence of the gene encoding aquaporin of Pharbitis nil Choisy (PnPIPl)

Creator:

Dąbrowska, Grażyna ; Mordaka, Paweł Mateusz

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

2008

Subject and Keywords:

biotechnologia

Abstract:

Aquaporins are membrane proteins that facilitate water transport across the membranes in various microorganisms, plants and animals. Plant aquaporins are divided into four groups based on the amino acid sequence similarities and intracellular localization; plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-like intrinsic proteins (NIPs) and small basic intrinsic proteins (SIPs). We found 35 EST sequences homologous to 3’ and 5’ termini of the partial cDNA of P. nil in the GenBank NCBl database. cDNA encoding full length aquaporin of P. nil was cloned with the use of the reverse transcription-polymerase chain reaction (RT-PCR). The 1189 bp full length cDNA sequence of aquaporin P. nil {PnPIPl} was obtained. Analysis of protein hydropathy indicated that cloned part of PnPIPl contained the NPA motif (Asn-Pro-Ala) that is present in all known aquaporins. The amino acid sequence of the PnPIPl protein exhibits 90, 89 and 88% sequence similarity to Petunio x hybrida, Nicotiana excelior and Fraxinus excelsior aquaporins respectively. We showed that the EST database is a useful tool for identification of the complete cDNA of known genes.

Relation:

Biotechnologia, vol.81, 2 (2008)-.

Volume:

81

Issue:

2

Start page:

190

End page:

198

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-76

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2008

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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