Tytuł:

DNA protein flow cytometry of dissociated cultures of human anaplastic gliomas. Pattern of proliferation and differentiation and the effect of a new cytostatic drug cladribine (2-CdA)

Inny tytuł:

Badania zdysocjowanych hodowli ludzkich złośliwych guzów glejopochodnych metodą cytometrii przepływowej DNA-bialko. Obraz proliferacji i różnicowania komórek oraz wpływ nowego leku cytostatycznego cladribine (2-CdA)

Twórca:

Grieb, Paweł ; Skierski, Janusz ; Jagielski, Janusz ; Ryba, Mirosław ; Mossakowski, Mirosław Jan (1929–2001)

Wydawca:

Medical Publishing House

Miejsce wydania:

Warsaw

Data wydania/powstania:

1995

Typ obiektu:

Journal/Article

Temat i słowa kluczowe:

glial tumors ; cladribine (2-CdA) ; DNA-protein cytometry

Abstrakt:

A technique of protein-DNA flow cytometry was applied to characterize cell cycling, and to assess the cytotoxicity of cladribine (2-chloro-2’deoxyadenosine) toward seven dissociated cultures of human primary brain tumors (anaplastic astrocytoma and glioblastoma multiforme) grown in vitro from surgical biopsies. Control cytograms were suggestive of that a clonogeneic fraction of the cell population consists mainly of cells with low protein content, which do not require increase in protein content before entering the S phase of the cell cycle. Following 24 or 48 hours exposure to cladribine, 1 nM -1 M, no cytotoxic effect was evident in 4 cultures, whereas in two cases dose-dependent progressive block of the phaseof the cell cycle was noted. In one case a massive cytotoxic effect resulted in disintegration of culture exposed to 100 nM ofthe drug. However, the treatment with cladribine was ineffective in a patient bearing the tumor which was the source forthe last culture, suggesting that cytotoxicity in vitro may not be predictive of clinical response.

Czasopismo/Seria/cykl:

Folia Neuropathologica

Tom:

33

Zeszyt:

3

Strona pocz.:

145

Strona końc.:

152

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

pdf

Język:

eng

Język streszczenia:

eng ; pol

Prawa:

Creative Commons Attribution BY 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitalizacja:

Mossakowski Medical Research Institute PAS

Lokalizacja oryginału:

Library of the Mossakowski Medical Research Institute PAS

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open