Title:

Gene cloning using subtractive hybridization

Subtitle:

Gene cloning using subtractive hybridization

Creator:

Rusin, Marek ; Szala, Stanisław

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1992

Subject and Keywords:

biotechnology

Abstract:

Genes expressed in temporally, environmentally or tissue specific way can be cloned using subtractivehybridization. It involves hybridization of single stranded cDNA from one cell type with mollar excess ofpolyA+RNAfrom another cell type. Unhybridized, cell specific cDNA is subsequently used or as subtractiveprobe or as material for construction of subtractive cDNA library. Modifications of this basic strategy arealso discussed.

Relation:

Biotechnologia, vol.17, 2 (1992)-.

Volume:

17

Issue:

2

Start page:

26

End page:

35

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-13

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open