Title:

Methods for isolation, purification and renaturation of recombinant proteins obtained in Escherichia coli bacteria

Subtitle:

Methods for isolation, purification and renaturation of recombinant proteins obtained in Escherichia coli bacteria

Creator:

Szczepanek, Andrzej ; Płucienniczak, Andrzej

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1994

Subject and Keywords:

biotechnology

Abstract:

Escherichia coli is the most useful bacterial species applied to genetic engineering in recombinant proteins production process. The supply of many polipeptides whith potential clinical orindustrial use is often limited by their low natural availability. Overexpressed polipeptides mayeither be located in the cytoplasm and periplasm of E. coli or secreted through the cell membraneinto the growth medium. Foreign proteins can be expressed in E. coli cells directly or as fusionproteins with prokaryotic sequences. Frequently, the overexpressed proteins acumulate in thebacterial cytoplasm or periplasm in the form of insoluble inclusion bodies.This review considers isolation, purification, solubilization and renaturation of recombinantproteins from E. coli cells, whieh is still a serious methodological and technical problem.

Relation:

Biotechnologia, vol.27, 4 (1994)-.

Volume:

27

Issue:

4

Start page:

102

End page:

125

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-23

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open