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Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:High expression yield and high volumetric productivity of fermentations employing recombinant E. coli need special processing. For recombinat protein production two strategies can beapplied. One is to carry out one step fermentation with simultaneous gene expression andelimination of vector-less segregants. The other is to carry out two step fermentation, in whichthe first step is the cell biomass production without gene expression followed by the inductionof gene expression in the second step. In this review different methods of controlling of recombinant gene expression, such as antibiotic pressure, specific mutant application, immobilizedcell bioreactors, selective cell recyrculation, chemical regulations, the use of temperature andoxygen sensitive promoters are presented.
Relation:Biotechnologia, vol.28, 1 (1995)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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