Metadata language
Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma
Subtitle:Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma
Creator: Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Type of object: Subject and Keywords: Abstract:Interferon gamma is a molecule which may serve as a good object for studying and explainingthe role of expression limiting conditions.Three levels of limiting factors modifications, i. e. operon choice and structure, changes inthe secondary mRNA structure, and the influence of post-translational modulation processes aredescribed. The relationship between the operon stucture and expression level for other heterologous proteins is compared with that of IFN-gamma. In particular, the shape of ribosome bindingsite (RBS) region and its affected different nucleotide substitutions are presented.The rule of N-terminal and C-terminal end changes for protein stability and inclusion bodyformation is also shown.
Relation:Biotechnologia, vol.37, 2 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Projects co-financed by: Access: