Tytuł:

Purification and some characteristics of Penicillium citrinum lipase

Inny tytuł:

Purification and some characteristics of Penicillium citrinum lipase

Twórca:

Maliszewska, Irena H.

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

1997

Typ obiektu:

Journal/Article

Temat i słowa kluczowe:

biotechnology

Abstrakt:

An extracellular lipase (glycerol ester hydrolases E.C. 3.1.1.3.) was isolated from a culturefiltrate of Penicillium citńnum. The purification procedure included ammonium sulfate precipitation, ultrafiltration and chromatography on Octyl-Sepharose CL-4B. The enzyme was 400-foldpurified with 9.66% yield. The molecular weight has been estimated by polyacrylamide gel electrophoresis under denaturing conditions at 26 000. On the other hand, lipase forms active dimersand tetramers aggregates as observed after native PAGE. Lipase from Penicillium citrinum showeda preference for triacylglycerols. It is non-specific and hydrolyzes each of the three ester bondsof triacylglycerols. The enzyme showed a maximum activity at pH 7.2 at 30°C and was stablein the range of pH 6.0-7.5 and the temperature of 10°C - 40°C.

Czasopismo/Seria/cykl:

Biotechnologia, vol.38, 3 (1997)-.

Tom:

38

Zeszyt:

3

Strona pocz.:

109

Strona końc.:

120

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open