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Nowak, Elżbieta ; Rojek, Żaneta ; Kucharska, Danuta ; Orlikowska, Teresa
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:An efficient method for genetic Agrobacterium tumejaciens-mediated transformation of fivecultivars Gerbera hybrida was established. The youngest leaves and shoot tips from proliferatingin vitro cultures were co-cultivated with disarmed strain LBA 4404/pBI121 carrying the chimaericgenes nptll and gus. The inoculated explants were repeatedly cultured on regeneration mediumwith kanamycin and Biotaksym. After 9-10 months from 0.04 to 0.25 independently transformedshoots from one inoculated leaf and from 0.06 to 0.65 from one inoculated shoot tip wereobtained, depending on cultivar and additional treatment. Transformed shoots accounted for 5.0to 50.0% of all regenerated shoots. Additional wounding of leaves before inoculation by squeezingand scratching off increased transformation efficiency. Better results for recalcitrant to regeneration cultivars were obtained if squeezed shoot tips were inoculated. Regenerating shoots wereselected on kanamycin, screened for gas expression with X-gluc and tested by PCR for nptlland gus genes. All together 162 transgenic shoots were obtained in cvs Amber, Boy, Ferrari,Mariola and Tamara, but only 24 relatively stable lines were established.
Relation:Biotechnologia, vol.39, 4 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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