Title:

In vitro culture of isolated embryos and ovules from wide crosseswithin the genus Brassica

Subtitle:

In vitro culture of isolated embryos and ovules from wide crosseswithin the genus Brassica

Creator:

Wojciechowski, Andrzej ; Maślankiewicz, Janetta ; Chen, Yuping ; Kwapiszewska, Miła ; Springer, Błażej

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2001

Subject and Keywords:

biotechnology

Abstract:

In vitro culture of hybrid embryos and ovules from crosses of B. campestris,B. oleracea, B. fruticulosa and B. napus were conducted on White, Murashige andSkoog, Murashige and Skoog modified by Keller and Nitsh and Nitsh media. Thehighest efficiency of crosses was obtained in the crosses B. campestris xB. oleracea, and the lowest in crosses with B. fruticulosa. In cultures of 572 embryos from 11 cross combinations, plants were regenerated from 24,5% of cultured embryos. From 3 cross combinations 502 ovules were placed on the medium. In this case the plants were regenerated from 2 of 3 crosses and the efficiency of the culture was over 46%.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

86

End page:

89

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open