Metadata language
Krótkie interferujące RNA jako narzędzia do sekwencyjno-specyficznego wyciszania ekspresji genów
Subtitle:Short interfering RNAs as tools for sequence-specific gene silencing
Creator:Sierant, Małgorzata ; Nawrot, Barbara
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:In diverse eukaryotes, dsRNA triggers the destruction of mRNA sharing thesame sequence as the dsRNA in the process called RNAi. The guides for sequence-specific degradation of mRNA are 21 nt short interfering RNAs (siRNAs).Synthetic siRNAs can efficiently mediate RNAi, but a drawback of RNAi is itstransient nature as a result of the limited availability and stability of syntheticoligonucleotides. Recently, several groups reported the construction of expression plasmid vectors that mediate the production of siRNAs under control ofPol III promoters. These vectors allow the continued expression of siRNAs in thecells resulting in persistent and specific suppression of target genes. The retroviralsiRNA expressing system allows for stable inactivation of the genes in primarycells or living organisms.
Relation:Biotechnologia, vol.61, 2 (2003)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Projects co-financed by: Access: