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Krioprezerwacja zawiesin komórkowych wybranych gatunkówz rodzaju Gentiana
Subtitle:Cryopreservation of cell suspensions of selected Gentiana
Creator:Mikuła, Anna ; Rybczyński, Jan J.
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures withnon-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required.In this presentation, following subjects will be discussed: 1) description ofsuspension cultures of various gentians, 2) cell pretreatments for surviving thestress of low temperature, 3) rearrangement of cell cytoplasm because of highsucrose concentrations, 4) the role of sucrose in the development of freezingtolerance and surviving of gentaiana suspensions, 5) the effect of strongplasmolysis induced by 3 M glucose.
Relation:Biotechnologia, vol.62, 3 (2003)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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