Tytuł:

Micropropagation of Miscanthus x giganteus (Greef & Deu.) from inflorescence explants

Inny tytuł:

Micropropagation of Miscanthus x giganteus (Greef & Deu.) from inflorescence explants

Twórca:

Głowacka, Katarzyna ; Zenkteler, Maciej ; Jeżowski, Stanisław

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2004

Temat i słowa kluczowe:

biotechnology

Abstrakt:

2 mm explants of immature inflorescences (0,5-3 cm) produced calluses on MS medium with 0,5 mg x M BAP and 2,5 mg x M 2,4-D. The surface sterilization in 10% sodium hypochloride for 20 min was successful in 74%. After 20 days of incubation, the surfaces of all explants were covered by callus. After 2 months of culture, from most of calluses embryo-like structures developed. The highest frequency of fully regenerated plants was observed on MS medium supplemented with 1-2 mg X |-> BAP. The regeneration rate of the cultured calluses for the first two weeks in 16 h day fotoperiod was twice as high as the one under a continuous light. Only two out of 6 thousand cultured mature spikelets produced calluses from which 574 plants were obtained after multiplication. Most of the plants obtained from the explants representing immature as mature inflorescences survived the transfer into soil in the greenhouses.

Czasopismo/Seria/cykl:

Biotechnologia, vol.65, 2 (2004)-.

Tom:

65

Zeszyt:

2

Strona pocz.:

251

Strona końc.:

259

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf ; application/pdf

Identyfikator zasobu:

0860-7796

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open