Tytuł:

Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation

Inny tytuł:

Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation

Twórca:

Stopa, Magdalena ; Dulak, Józef ; Józkowicz, Alicja

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2007

Temat i słowa kluczowe:

biotechnology

Abstrakt:

Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene p-galactosidase (Adp-gal). We optimised the adsorption time and dose of Adp-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 lU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.

Czasopismo/Seria/cykl:

Biotechnologia, vol.78, 3 (2007)-.

Tom:

78

Zeszyt:

3

Strona pocz.:

123

Strona końc.:

140

Typ zasobu:

Text

Szczegółowy typ zasobu:

Journal

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-74

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open