@misc{Skrzyszowska_Maria_The_2006,
 author={Skrzyszowska, Maria and Samiec, Marcin},
 volume={72},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.72, 1 (2006)-.},
 howpublished={online},
 year={2006},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Somatic cell nuclear transfer (SCNT) technique in pigs remains relatively low (2% to 5% of produced piglets), that is why further efforts have to be made to optimize both a multi-step cloning procedure and to improve a structu- ro-functiona! quality of recipient oocytes and nuclear donor cells. Pre- and postimplantation developmental potential of porcine SCNT-derived embryos depends to a high degree on not only coordination of mitotic cycle stage with phenotype of nuclear donor cell, but also proper combination of the methods of maternal chromosome elimination (enucleation), oocyte reconstruction techniques, the systems of artificial activation of generated nuclear-cytoplasmic hybrids (clonal cybrids) and in vitro culture of reconstructed embryos. Generally, it can result in increasing the competences of both somatic nuclear and mitochondrial genome for epigenetic remodeling/reprogramming in developing cloned embryos.},
 title={The use of new strategies in the studies on somatic cell cloning in pigs},
 type={Text},
 URL={http://www.rcin.org.pl/Content/87786/PDF/POZN271_114740_biotechnologia-2006-no1-skrzyszowska.pdf},
 keywords={biotechnology},
}