@misc{Stopa_Magdalena_Optimization_2007,
 author={Stopa, Magdalena and Dulak, Józef and Józkowicz, Alicja},
 volume={78},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.78, 3 (2007)-.},
 howpublished={online},
 year={2007},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene p-galactosidase (Adp-gal). We optimised the adsorption time and dose of Adp-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 lU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.},
 type={Text},
 title={Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation},
 URL={http://www.rcin.org.pl/Content/78588/PDF/POZN271_102561_biotechnologia-2007-nr3-stopa.pdf},
 keywords={biotechnology},
}