@misc{Gołda_Sławomir_Regulation_2007,
 author={Gołda, Sławomir and Kucharzewska, Paulina and Cisowski, Jarosław and Florczyk, Urszula and Zagórska, Anna and Jaźwa, Agnieszka and Łoboda, Agnieszka and Józkowicz, Alicja and Dulak, Józef},
 volume={78},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.78, 3 (2007)-.},
 howpublished={online},
 year={2007},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Regulation of gene expression in gene therapy is crucial for obtaining the therapeutic effects, thanks to limitation of transgene activity to the selected cells in a given time. In this paper we have focused on plasmid expression systems regulated by doxycycline or hypoxia. We have described in details the structure, regulatory elements and biological applications of 1) the modified, commercially available Tet-On system, expressing doxycycline-controlled P-galactosidase and 2) hypoxia-activated FGF-4A^GF expression plasmid containing the hypoxia responsive sequence. The presented expression systems can also be used in viral vectors, enabling not only regulated, but also high and long-term expression of transgenes.},
 type={Text},
 title={Regulation of gene expression in plasmid vectors: doxocyclin-de- pendent and hypoxia-regulated systems},
 URL={http://www.rcin.org.pl/Content/76141/PDF/POZN271_100057_biotechnologia-2007-nr3-golda.pdf},
 keywords={biotechnology},
}