@misc{Grieb_Paweł_DNA_1995,
 author={Grieb, Paweł and Skierski, Janusz and Jagielski, Janusz and Ryba, Mirosław and Mossakowski, Mirosław Jan (1929–2001)},
 volume={33},
 number={3},
 copyright={Creative Commons Attribution BY 4.0 license},
 address={Warsaw},
 journal={Folia Neuropathologica},
 howpublished={online},
 year={1995},
 publisher={Medical Publishing House},
 language={eng},
 abstract={A technique of protein-DNA flow cytometry was applied to characterize cell cycling, and to assess the cytotoxicity of cladribine (2-chloro-2’deoxyadenosine) toward seven dissociated cultures of human primary brain tumors (anaplastic astrocytoma and glioblastoma multiforme) grown in vitro from surgical biopsies. Control cytograms were suggestive of that a clonogeneic fraction of the cell population consists mainly of cells with low protein content, which do not require increase in protein content before entering the S phase of the cell cycle. Following 24 or 48 hours exposure to cladribine, 1 nM -1 M, no cytotoxic effect was evident in 4 cultures, whereas in two cases dose-dependent progressive block of the phaseof the cell cycle was noted. In one case a massive cytotoxic effect resulted in disintegration of culture exposed to 100 nM ofthe drug. However, the treatment with cladribine was ineffective in a patient bearing the tumor which was the source forthe last culture, suggesting that cytotoxicity in vitro may not be predictive of clinical response.},
 type={Text},
 title={DNA protein flow cytometry of dissociated cultures of human anaplastic gliomas. Pattern of proliferation and differentiation and the effect of a new cytostatic drug cladribine (2-CdA)},
 URL={http://www.rcin.org.pl/Content/71856/PDF/publ_247.pdf},
 keywords={glial tumors, cladribine (2-CdA), DNA-protein cytometry},
}