@misc{Karssen_Gerrit_Pratylenchus_2000,
 author={Karssen, Gerrit and Waeyenberge, Lieven and Moens, Maurice},
 editor={Polska Akademia Nauk. Muzeum i Instytut Zoologii},
 copyright={Rights Reserved - Restricted Access},
 address={Warszawa},
 howpublished={online},
 year={2000},
 language={eng},
 abstract={A root-lesion nematode, Pratylenchus brzeskiisp. nov., is described and illustrated from the roots of Ammophila arenaria (L.). Link from the coastal dunes of the Netherlands; this new species is morphologically characterized by: relatively long females (625–735 μm) with two lip annuli, long stylet (+19 μm) with broad stylet knobs, an teriorly slightly indented, short DGO; pharyngeal gland lobe ranging from 65 to 100 μm; four lateral lines present, inner lines closely together; spermatheca faint, oval to rectangular shaped; vulva posterior, ranging from 75 to 78 %, lips slightly protruding, posterior uterine sac 19 to 35 μm long; tail conoid, terminus smooth, rounded to narrowly rounded, relatively long hyaline tail part present. Males occurred abundantly and are slightly smaller in most morphological characteristics. PCR was used to amplify the internal transcribed spacer regions of aribosomal DNA-cluster of P. brzeskiisp. nov. and compared with the morphological related species P. coffeae, P. loosiand P. penetrans. The rDNA fragments were cut with restriction enzymes; interspecific RFLP’s were observed. Pratylenchus brzeskiisp. nov. was also isolated from the coastal dune grasses Elymus farctus (Viv.) Meldrisand Leymus arenarius Hochst., and detected in coastal dunes of Belgium, France and Poland.},
 type={Text},
 title={Pratylenchus brzeskii sp. nov. (Nematoda:Pratylenchidae), a root-lesion nematode from European coastal dunes},
 volume={50},
 number={2},
 journal={Annales Zoologici},
 publisher={Muzeum i Instytut Zoologii PAN},
 keywords={Ammophila arenaria, coastal dunes, PCR-RFLP, Pratylenchus brzeskii, ribosomal DNA, taxonomy},
}