@misc{Marecik_Roman_Sposoby_1998,
 author={Marecik, Roman and Króliczak, Paweł},
 volume={40},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.40, 1 (1998)-.},
 howpublished={online},
 year={1998},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Tissue culture has been utilized for producing virus free plants for vegetative propagationand genetic engineering. In addition, altered cells lines showing higher productivity of secondarymetabolities can be obtained using tissue culture. The wide use of tissue culture requires thedevelopment of new preservation techniques for in vitro culture material.One of this method is growth reduction achieved by modifying various parameters such as:temperature, culture medium, gasous environment. However, crypreservation (i. e. storage inliquid nitrogen -196°C) is the only method available nowadays for long-term conservation. Newcryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccationhelped expend the list of plant species that can tolerate low temperatures and are characterizedby a normal rate of growth. Each step of the cryopreservation procces requires specific conditions},
 type={Text},
 title={Sposoby przechowywania materiału roślinnego w kulturach in vitro},
 URL={http://www.rcin.org.pl/Content/144628/PDF/POZN271_180064_biotechnologia-1998-no1-merecik.pdf},
 keywords={biotechnology},
}