@misc{Piotrowska_Karolina_Wielokrotne_1998, author={Piotrowska, Karolina and Modliński, Jacek A.}, volume={41}, number={2}, copyright={Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0}, journal={Biotechnologia, vol.41, 2 (1998)-.}, howpublished={online}, year={1998}, publisher={Komitet Biotechnologii PAN}, publisher={Instytut Chemii Bioorganicznej PAN}, language={pol}, abstract={The main purpose of nuclear transfer in domestic species is to produce a large number ofidentical animals. There are two main ways to produce clones by nuclear transfer. One is touse the ICM and ED cells cultured under special conditions, as donor nuclei. The other way isto use the nuclear transfer embiyo itself as the donor for the next generation of cloning (multiplegenerational cloning). The in vitro and in vivo developmental ability of nuclear transferred embryosis the same in the case of the first three generations. A limited number of multiple-generationclones were transferred into recipient heifers, resulting in offspring from 1, 11 and 111 generationclones. The strategy to increase the efficiency of multiple generational bovine embryo cloning isdiscussed as well as the possible use of rabbit embryos as an experimental model.}, title={Wielokrotne klonowanie zarodków zwierząt gospodarskich}, type={Tekst}, URL={http://www.rcin.org.pl/Content/144334/PDF/POZN271_179648_biotechnologia-1998-no2-piotrowska.pdf}, keywords={biotechnologia}, }