@misc{Szramka_Bożena_Thiol-activated_2001,
 author={Szramka, Bożena and Kurlenda, Julianna and Podhajska, Anna J.},
 volume={54},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.54, 3 (2001)-.},
 howpublished={online},
 year={2001},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The article contains information regarding the similarities and differencesbetween proteins of the thiol-activated cytolysins family (TACY). Members ofthis group of haemolysins are produced by several species of Gram-positive bacteria \{Listeria spp., Streptococcus spp., Bacillus spp., Clostridium spp.,) and also byGram-negative Klebsiella spp. Their cytolytic activity is sensitive to oxygen andcan be restored by adding reducing compounds. TACY bind to cholesterol-containing membranes to form pores. Preincubation of the toxin with smallamounts of cholesterol inhibits hemolytic activity as well as cytolysis ofeucaryotic cells. Members ofthe group show 40-70% similarity in amino acid sequence, and contain an almost invariant Trp-rich undecapeptide sequence(ECTGLAWEWWR). The TACY are important virulence factors. Recently increased use has been made of molecular methods (PCR, hybridization) in the detection and identification of the TACY producing bacteria.},
 title={Thiol-activated hemolysins as virulence markers of bacteria},
 type={Text},
 URL={http://www.rcin.org.pl/Content/138884/PDF/POZN271_174177_biotechnologia-2001-no3-szramka.pdf},
 keywords={biotechnology},
}