@misc{Zuzga_Sabina_Generation_2003, author={Zuzga, Sabina and Burza, Wojciech and Malepszy, Stefan}, volume={63}, number={4}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.63, 4 (2003)-.}, howpublished={online}, year={2003}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Since the first reports of successful plant transformation appeared, therehave been steady improvements of the transformation methods. Nowadays,transgenic plants without the incorporation of selection genes for antibiotic orherbicide resistance would be the only ones acceptable to the public, so elimination of these genes from transgenic crops prior to their field release and commercialization seems inevitable. Several strategies have been developed to generate marker-free transgenic plants, including: positive selection, alternativemarker genes, co-transformation, site-specific recombination, transpozon-mediated approaches and intrachromosomal homologous recombination. The efficiency of these methods is various as comparing to the traditional marker assisted selection - higher in case of alternative procedures and lower in others.}, title={Generation of selectable marker-free transgenic plants}, type={Text}, URL={http://www.rcin.org.pl/Content/129579/PDF/POZN271_162416_biotechnologia-2003-no4-zuzga.pdf}, keywords={biotechnology}, }