@misc{Grzegorczyk_Izabela_Micropropagation_2004, author={Grzegorczyk, Izabela and WysokiƄska, Halina}, volume={65}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.65, 2 (2004)-.}, howpublished={online}, year={2004}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 pmol/1 lAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 pmol/1 was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was 1/2 MS agar medium without growth regulators.}, title={Micropropagation of Salvia officinalis L. by shoot tips}, type={Text}, URL={http://www.rcin.org.pl/Content/122751/PDF/POZN271_153182_biotechnologia-2004-no2-grzegorczyk.pdf}, keywords={biotechnology}, }