@misc{Szybka-Hrynkiewicz_Przemysław_Induction,_2004,
 author={Szybka-Hrynkiewicz, Przemysław and Janeczko, Zbigniew},
 volume={66},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.66, 3 (2004)-.},
 howpublished={online},
 year={2004},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={In this study, we examined the effect of; coconut water (CW), plant growth regulators NAA - a-naphthaleneacetic acid, IBA - indole-3-butyric acid, 2,4-D-2,4-dichlorophenoxyacetic acid as well as 6-BAP - 6-benzyl amintpurine and steroidal hormones (progesterone, estriol, estrone, 17-p-estradiol, andro.terone, androstendione) on callus of Polygonatum verticillatum (L.) All. induction The best callus formation was obtained from the hypocotyl part ol plants cultivated from the seeds after their stratification (two weeks in 4°C)on the Murashige and Skoog (MS) medium, which contained: 100 mg/cm^ of myoinositol, 30 g/dm^ of sucrose, 2 mg/dm^ of 2,4-D and 1 pM/lm^ of 17-p-estradiol. Additionally, the efforts to obtain the callus from underground parts of P. verticillatum were made. It was done on MS medium conaining: 1 mg/dm^ 2,4-D, 1 pM/dm^ 17-p-estradiol and 2 ing/dm^O - BAP. The callu; grown in in vitro conditions was used for the fitochemical analysis to determne the presence of diosgenin.},
 type={Text},
 title={Induction, proliferation and fitochemical analysis of callus tis»ue of Polygonatum verticillatum (L.) All.},
 URL={http://www.rcin.org.pl/Content/119512/PDF/POZN271_148828_biotechnologia-2004-no3-szybka.pdf},
 keywords={biotechnology},
}