@misc{_Production_2007,
 volume={78},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 howpublished={online},
 year={2007},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The paper presents the production of adenoviral vectors (AdV) containing P-galactosidase (Adp-gal), from the transfer of recombinant viral DNA into packing cell line (HEK293) to the titration of viral particles. Optimisation of the methods (preparation of DNA for transfection, adsorption time during the infection of cells, amount of serum in the medium, time-point of vector isolation) enables obtaining a titer of up to 10'° lU/mL. Adp-gal were titrated with several methods, with P-gal in situ staining used as a reference. We found that the most suitable titration method of the vectors containing other than reporter genes was the Rapid Titer ELISA kit™.},
 title={Production of adenoviral vectors of the first generation - optimization of the method},
 type={Text},
 URL={http://www.rcin.org.pl/Content/76143/PDF/POZN271_100059_biotechnologia-2007-nr3-stopa.pdf},
 keywords={biotechnology},
}