@misc{Samiec_Marcin_Artificial_2009, author={Samiec, Marcin}, volume={86}, number={3}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.86, 3 (2009)-.}, howpublished={online}, year={2009}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={In the somatic cell cloning of pigs, nuclear transfer-derived oocytes are artificially stimulated with the use one of the three experimental protocols; 1) electrical, chemical or physicochemical delayed activation (i.e., post-activation); 2) simultaneous fusion and electrical activation (SF-EA) or simultaneous electrofusion and physicochemical activation, as well as 3) chemical sequential (combined) electrical and chemical activation.}, title={Artificial activation of porcine oocytes in somatic cell cloning procedure - biotechnological, genetic and epigenetic aspects}, type={Text}, URL={http://www.rcin.org.pl/Content/71972/PDF/POZN271_94415_biotechnologia-2009-no3-samiec.pdf}, keywords={biotechnology}, }