@misc{Antczak_Tadeusz_Entrapment_2000,
 author={Antczak, Tadeusz and Bugla, J. and Mastalerz, M. and Niemiec, A. and Szeja, W. and Ślęk, M. and Galas, E.},
 volume={51},
 number={4},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.51, 4 (2000)-.},
 howpublished={online},
 year={2000},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Lipases Mucor circinelloides and Mucor racemosus immobilised in situ were closed in microporous polysacharides hydrophilic gel cross-linked using a solutionof calcium salts. In order to increase the porosity of polysacharides matrix during its cross-linking oligomer molecules of ethylene oxide (optimal Polikol1000) were incorporated in the structure of the matrix. Then the oligomer wasremoved by acetone extraction. The obtained biocatalyst preparations were tested in hydrolysis of esters and esterification of oleic acid with butanol. The hydrolysis was carried out in water saturated organic solvents medium (n-hexaneand diisopropylether). It was found that the efficiency of M. racemosus lipase immobilisation in hydrolysis of n-butyl oleate, n-butyl palmitate, n-butyl stearate,n-butyl laurate amounted to 60%. The efficiency of M. circinelloides lipase immobilisation in esterification of oleic acid with 1-butanol in hexane achieved 45%},
 title={Entrapment of immobilised in situ intracellular lipase of Mucor in microporous capsules},
 type={Text},
 URL={http://www.rcin.org.pl/Content/140301/PDF/POZN271_175651_biotechnologia-2000-no4-antczak.pdf},
 keywords={biotechnology},
}