@misc{Szewczyk_Krzysztof_W._Puriflcation_2002,
 author={Szewczyk, Krzysztof W. and Orzeszak, Małgorzata},
 volume={57},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.57, 2 (2002)-.},
 howpublished={online},
 year={2002},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Methods of purification of chitin deacetylase are discussed. A two stepmethod of purification of chitin deacetylase from mycelial extracts ofthe fungusAbsidia orchidis by chromatography is presented. The crude enzyme extract waspurified by a gel chromatography and then by ion exchange chromatography.Specific activity of purified enzyme was 12.3 U/mg and final purification degreewas 147. The apparent molecular mass of the enzyme was 75 kDa. When 0 -hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pHfor enzyme activity was 5,5 and the optimum temperature was 50°C},
 title={Puriflcation and properties of chitin deacetylase from Absidiaorchidis},
 type={Text},
 URL={http://www.rcin.org.pl/Content/138077/PDF/POZN271_173239_biotechnologia-2002-no2-szewczyk.pdf},
 keywords={biotechnology},
}